Fig. 8
Transcriptomics analysis. A RNA sequencing was performed to identify differentially expressed genes in lung tissue samples from rabbits. Principal components analysis was conducted to evaluate genomic similarities of different groups. B Number of differentially expressed genes in each ventilation group vs. the control group. C, D Volcano plots of differentially expressed genes in 10 cmH2O group vs. control group (C) and 20 cmH2O group vs. 10 cmH2O group (D). E Venn diagram of the numbers of differentially expressed genes. F, G Heatmaps of differentially expressed genes in 10 cmH2O group vs. control group (F) and 20 cmH2O group vs. 10 cmH2O group (G). H Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially expressed genes in 25 cmH2O group vs. 10 cmH2O group. DP: driving pressure; PCA: principal components analysis