Fig. 5
High driving pressure aggravated ALI. A Rabbit lung tissue samples were collected at 7 h after ventilation and stained with hematoxylin and eosin. Images were acquired at magnification ×200. B Bronchoalveolar lavage fluid was obtained, and protein concentration was measured. C Lung injury scores were calculated in 20 high-power fields per animal. D Lung tissue was immediately weighed after collection and reweighted after drying at 80 °C for 48 h. The wet/dry ratio was calculated. Data are presented as the means ± SD. #P < 0.05 vs. control; †P < 0.05 vs. 10 cmH2O; n = 5. W/D: wet-to-dry weight ratio; DP: driving pressure; BALF: bronchoalveolar lavage fluid